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Plos One : an Integrated in Silicoapproach to Design Specific Inhibitors Targeting Human Polya-specific Ribonuclease, Volume 7

By Donald, Bruce, R.

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Book Id: WPLBN0003933735
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : an Integrated in Silicoapproach to Design Specific Inhibitors Targeting Human Polya-specific Ribonuclease, Volume 7  
Author: Donald, Bruce, R.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Donald, B. R. (n.d.). Plos One : an Integrated in Silicoapproach to Design Specific Inhibitors Targeting Human Polya-specific Ribonuclease, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : Poly(A)-specific ribonuclease (PARN) is an exoribonuclease/deadenylase that degrades 39-end poly(A) tails in almost all eukaryotic organisms. Much of the biochemical and structural information on PARN comes from the human enzyme. However, the existence of PARN all along the eukaryotic evolutionary ladder requires further and thorough investigation. Although the complete structure of the full-length human PARN, as well as several aspects of the catalytic mechanism still remain elusive, many previous studies indicate that PARN can be used as potent and promising anti-cancer target. In the present study, we attempt to complement the existing structural information on PARN with in-depth bioinformatics analyses, in order to get a hologram of the molecular evolution of PARNs active site. In an effort to draw an outline, which allows specific drug design targeting PARN, an unequivocally specific platform was designed for the development of selective modulators focusing on the unique structural and catalytic features of the enzyme. Extensive phylogenetic analysis based on all the publicly available genomes indicated a broad distribution for PARN across eukaryotic species and revealed structurally important amino acids which could be assigned as potentially strong contributors to the regulation of the catalytic mechanism of PARN. Based on the above, we propose a comprehensive in silico model for the PARN’s catalytic mechanism and moreover, we developed a 3D pharmacophore model, which was subsequently used for the introduction of DNP-poly(A) amphipathic substrate analog as a potential inhibitor of PARN. Indeed, biochemical analysis revealed that DNPpoly( A) inhibits PARN competitively. Our approach provides an efficient integrated platform for the rational design of pharmacophore models as well as novel modulators of PARN with therapeutic potential.

 

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