World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Cd44+ Cancer Stem-like Cells in Ebv-associated Nasopharyngeal Carcinoma, Volume 7

By Loeb, David

Click here to view

Book Id: WPLBN0003934775
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Cd44+ Cancer Stem-like Cells in Ebv-associated Nasopharyngeal Carcinoma, Volume 7  
Author: Loeb, David
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Publication Date:
Publisher: Plos


APA MLA Chicago

Loeb, D. (n.d.). Plos One : Cd44+ Cancer Stem-like Cells in Ebv-associated Nasopharyngeal Carcinoma, Volume 7. Retrieved from

Description : Nasopharyngeal carcinoma (NPC) is a unique EBV-associated epithelial malignancy, showing highly invasive and metastatic phenotype. Despite increasing evidence demonstrating the critical role of cancer stem-like cells (CSCs) in the maintenance and progression of tumors in a variety of malignancies, the existence and properties of CSC in EBV-associated NPC are largely unknown. Our study aims to elucidate the presence and role of CSCs in the pathogenesis of this malignant disease. Sphere-forming cells were isolated from an EBV-positive NPC cell line C666-1 and its tumor-initiating properties were confirmed by in vitro and in vivo assays. In these spheroids, up-regulation of multiple stem cell markers were found. By flow cytometry, we demonstrated that both CD44 and SOX2 were overexpressed in a majority of sphere-forming C666-1 cells. The CD44+SOX2+ cells was detected in a minor population in EBV-positive xenografts and primary tumors and considered as potential CSC in NPC. Notably, the isolated CD44+ NPC cells were resistant to chemotherapeutic agents and with higher spheroid formation efficiency, showing CSC properties. On the other hand, microarray analysis has revealed a number of differentially expressed genes involved in transcription regulation (e.g. FOXN4, GLI1), immune response (CCR7, IL8) and transmembrane transport (e.g. ABCC3, ABCC11) in the spheroids. Among these genes, increased expression of CCR7 in CD44+ CSCs was confirmed in NPC xenografts and primary tumors. Importantly, blocking of CCR7 abolished the sphereforming ability of C666-1 in vitro. Expression of CCR7 was associated with recurrent disease and distant metastasis. The current study defined the specific properties of a CSC subpopulation in EBV-associated NPC. Our findings provided new insights into developing effective therapies targeting on CSCs, thereby potentiating treatment efficacy for NPC patients.


Click To View

Additional Books

  • Plos One : the Etramp Family Member Sep2... (by )
  • Plos One : Crawling at High Speeds ; Ste... (by )
  • Plos One : Enterococcus Faecalis Infecti... (by )
  • Plos One : High Cytoplasmic Foxo1 and Pf... (by )
  • Plos One : Sleep Promotes the Extraction... (by )
  • Plos One : Fcεr1-mediated Mast Cell Reac... (by )
  • Plos One : Rescue of Murine F508Del Cftr... (by )
  • Plos One : Virus-specific Immune Respons... (by )
  • Plos One : Dynamics of Cell-fate Determi... (by )
  • Plos One : Decrease of Fibulin-3 in Hepa... (by )
  • Plos One : Branched Motifs Enable Long-r... (by )
  • Plos One : Reactive Oxygen Species Modul... (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.