World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Cysteine Depletion Causes Oxidative Stress and Triggers Outer Membrane Vesicle Release by Neisseria Meningitidis; Implications for Vaccine Development, Volume 7

By Morici, Lisa, A.

Click here to view

Book Id: WPLBN0003935794
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Cysteine Depletion Causes Oxidative Stress and Triggers Outer Membrane Vesicle Release by Neisseria Meningitidis; Implications for Vaccine Development, Volume 7  
Author: Morici, Lisa, A.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Morici, L. A. (n.d.). Plos One : Cysteine Depletion Causes Oxidative Stress and Triggers Outer Membrane Vesicle Release by Neisseria Meningitidis; Implications for Vaccine Development, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : Outer membrane vesicles (OMV) contain immunogenic proteins and contribute to in vivo survival and virulence of bacterial pathogens. The first OMV vaccines successfully stopped Neisseria meningitidis serogroup B outbreaks but required detergent-extraction for endotoxin removal. Current vaccines use attenuated endotoxin, to preserve immunological properties and allow a detergent-free process. The preferred process is based on spontaneously released OMV (sOMV), which are most similar to in vivo vesicles and easier to purify. The release mechanism however is poorly understood resulting in low yield. This study with N. meningitidis demonstrates that an external stimulus, cysteine depletion, can trigger growth arrest and sOMV release in sufficient quantities for vaccine production (61500 human doses per liter cultivation). Transcriptome analysis suggests that cysteine depletion impairs iron-sulfur protein assembly and causes oxidative stress. Involvement of oxidative stress is confirmed by showing that addition of reactive oxygen species during cysteine-rich growth also triggers vesiculation. The sOMV in this study are similar to vesicles from natural infection, therefore cysteinedependent vesiculation is likely to be relevant for the in vivo pathogenesis of N. meningitidis.

 

Click To View

Additional Books


  • Plos One : Facial Morphogenesis of the E... (by )
  • Plos One : Resection Probability Maps fo... (by )
  • Plos One : Htra3 as an Early Marker for ... (by )
  • Plos One : Proteasome Inhibition Augment... (by )
  • Plos One : Where to Settle—settlement Pr... (by )
  • Plos One : Traditional Nets Interfere wi... (by )
  • Plos One : Graft-versus-host Disease is ... (by )
  • Plos One : the Subthalamic Microlesion S... (by )
  • Plos One : Methionine-restricted C57Bl, ... (by )
  • Plos One : Relation Between Mild to Mode... (by )
  • Plos One : Recombinant Human Endostatin ... (by )
  • Plos One : Probabilistic Mri Brain Anato... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.