World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Fission Yeast Nod1 is a Component of Cortical Nodes Involved in Cell Size Control and Division Site Placement, Volume 7

By Whitehall, Simon

Click here to view

Book Id: WPLBN0003937699
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Fission Yeast Nod1 is a Component of Cortical Nodes Involved in Cell Size Control and Division Site Placement, Volume 7  
Author: Whitehall, Simon
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Whitehall, S. (n.d.). Plos One : Fission Yeast Nod1 is a Component of Cortical Nodes Involved in Cell Size Control and Division Site Placement, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : Most cells enter mitosis once they have reached a defined size. In the fission yeast Schizosaccharomyces pombe, mitotic entry is orchestrated by a geometry-sensing mechanism that involves the Cdk1/Cdc2-inhibiting Wee1 kinase. The factors upstream of Wee1 gather together in interphase to form a characteristic medial and cortical belt of nodes. Nodes are also considered to be precursors of the cytokinesis contractile actomyosin ring (CAR). Here we describe a new component of the interphase nodes and cytokinesis rings, which we named Nod1. Consistent with its role in cell size control at division, nod1D cells were elongated and epistatic with regulators of Wee1. Through biochemical and localisation studies, we placed Nod1 in a complex with the Rho-guanine nucleotide exchange factor Gef2. Nod1 and Gef2 mutually recruited each other in nodes and Nod1 also assembles Gef2 in rings. Like gef2D, nod1D cells showed a mild displacement of their division plane and this phenotype was severely exacerbated when the parallel Polo kinase pathway was also compromised. We conclude that Nod1 specifies the division site by localising Gef2 to the mitotic cell middle. Previous work showed that Gef2 in turn anchors factors that control the spatio-temporal recruitment of the actin nucleation machinery. It is believed that the actin filaments originated from the nodes pull nodes together into a single contractile ring. Surprisingly however, we found that node proteins could form pre-ring helical filaments in a cdc12-112 mutant in which nucleation of the actin ring is impaired. Furthermore, the deletion of either nod1 or gef2 created an un-expected situation where different ring components were recruited sequentially rather than simultaneously. At later stages of cytokinesis, these various rings appeared inter-fitted rather than merged. This study brings a new slant to the understanding of CAR assembly and function.

 

Click To View

Additional Books


  • Plos One : Seasonal Changes in Endotoxin... (by )
  • Plos One : Surface Microstructures on Pl... (by )
  • Plos One : Educational Intervention Impr... (by )
  • Plos One : Genotyping-by-sequencing Gbs ... (by )
  • Plos One : Cyclosporin a Inhibits Rotavi... (by )
  • Plos One : Improved Flow Cytometric Asse... (by )
  • Plos One : Blood Cues Induce Antipredato... (by )
  • Plos One : Normal T Cell Selection Occur... (by )
  • Plos One : Anticipating the Prevalence o... (by )
  • Plos One : Prevalence of Human Papilloma... (by )
  • Plos One : Deletion of Sema3A or Plexina... (by )
  • Plos One : My Neighbor ; Children’s Perc... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.