World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : High Resolution Melting Analysis is a More Sensitive and Effective Alternative to Gel-based Platforms in Analysis of Ssr – an Example in Citrus, Volume 7

By Niedz, Randall P.

Click here to view

Book Id: WPLBN0003938534
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : High Resolution Melting Analysis is a More Sensitive and Effective Alternative to Gel-based Platforms in Analysis of Ssr – an Example in Citrus, Volume 7  
Author: Niedz, Randall P.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Niedz, R. P. (n.d.). Plos One : High Resolution Melting Analysis is a More Sensitive and Effective Alternative to Gel-based Platforms in Analysis of Ssr – an Example in Citrus, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : High resolution melting curve analysis (HRM) has been used as an efficient, accurate and cost-effective tool to detect single nucleotide polymorphisms (SNPs) or insertions or deletions (INDELs). However, its efficiency, accuracy and applicability to discriminate microsatellite polymorphism have not been extensively assessed. The traditional protocols used for SSR genotyping include PCR amplification of the DNA fragment and the separation of the fragments on electrophoresis-based platform. However, post-PCR handling processes are laborious and costly. Furthermore, SNPs present in the sequences flanking repeat motif cannot be detected by polyacrylamide-gel-electrophoresis based methods. In the present study, we compared the discriminating power of HRM with the traditional electrophoresis-based methods and provided a panel of primers for HRM genotyping in Citrus. The results showed that sixteen SSR markers produced distinct polymorphic melting curves among the Citrus spp investigated through HRM analysis. Among those, 10 showed more genotypes by HRM analysis than capillary electrophoresis owing to the presence of SNPs in the amplicons. For the SSR markers without SNPs present in the flanking region, HRM also gave distinct melting curves which detected same genotypes as were shown in capillary electrophoresis (CE) analysis. Moreover, HRM analysis allowed the discrimination of most of the 15 citrus genotypes and the resulting genetic distance analysis clustered them into three main branches. In conclusion, it has been approved that HRM is not only an efficient and cost-effective alternative of electrophoresis-based method for SSR markers, but also a method to uncover more polymorphisms contributed by SNPs present in SSRs. It was therefore suggested that the panel of SSR markers could be used in a variety of applications in the citrus biodiversity and breeding programs using HRM analysis. Furthermore, we speculate that the HRM analysis can be employed to analyse SSR markers in a wide range of applications in all other species.

 

Click To View

Additional Books


  • Plos One : In-situ Effects of Eutrophica... (by )
  • Plos One : the Aqueous Extract of Ficus ... (by )
  • Plos One : Determining Occurrence Dynami... (by )
  • Plos One : Comparative Analysis of Hepat... (by )
  • Plos One : Selective Ablation of Ctip2, ... (by )
  • Plos One : Measuring Social Contacts in ... (by )
  • Plos One : Health Risk Assessment of Hea... (by )
  • Plos One : Basic Amino Acid Mutations in... (by )
  • Plos One : Toxoplasma Gondii Sporozoites... (by )
  • Plos One : Automated Filtering of Intrin... (by )
  • Plos One : Impaired Virus Clearance, Com... (by )
  • Plos One : Processing of Continuously Pr... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.