World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Functional Complementation of a Model Target to Study Vpu Sensitivity, Volume 8

By Schindler, Michael

Click here to view

Book Id: WPLBN0003948747
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Functional Complementation of a Model Target to Study Vpu Sensitivity, Volume 8  
Author: Schindler, Michael
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Schindler, M. (n.d.). Plos One : Functional Complementation of a Model Target to Study Vpu Sensitivity, Volume 8. Retrieved from http://worldebookfair.com/


Description
Description : HIV-1 forms infectious particles with Murine Leukemia virus (MLV) Env, but not with the closely related Gibbon ape Leukemia Virus (GaLV) Env. We have determined that the incompatibility between HIV-1 and GaLV Env is primarily caused by the HIV-1 accessory protein Vpu, which prevents GaLV Env from being incorporated into particles. We have characterized the ‘Vpu sensitivity sequence’ in the cytoplasmic tail domain (CTD) of GaLV Env using a chimeric MLV Env with the GaLV Env CTD (MLV/GaLV Env). Vpu sensitivity is dependent on an alpha helix with a positively charged face containing at least one Lysine. In the present study, we utilized functional complementation to address whether all the three helices in the CTD of an Env trimer have to contain the Vpu sensitivity motif for the trimer to be modulated by Vpu. Taking advantage of the functional complementation of the binding defective (D84K) and fusion defective (L493V) MLV and MLV/GaLV Env mutants, we were able to assay the activity of mixed trimers containing both MLV and GaLV CTDs. Mixed trimers containing both MLV and GaLV CTDs were functionally active and remained sensitive to Vpu. However, trimers containing an Env with the GaLV CTD and an Env with no CTD remained functional but were resistant to Vpu. Together these data suggest that the presence of at least one GaLV CTD is sufficient to make an Env trimer sensitive to Vpu, but only if it is part of a trimeric CTD complex.

 

Click To View

Additional Books


  • Plos One : Statistical Analysis of Sleep... (by )
  • Plos One : Overnight Fasting Regulates I... (by )
  • Plos One : Effects of Hypoxic Exposure D... (by )
  • Plos One : Evaluation of Pacific White S... (by )
  • Plos One : Integrating Chemical Footprin... (by )
  • Plos One : Standard Addition Quantitativ... (by )
  • Plos One : a Novel Trans Conformation of... (by )
  • Plos One : Evidence That Life History Ch... (by )
  • Plos One : Heterologous Stop Codon Readt... (by )
  • Plos One : a Novel Scn9A Mutation Respon... (by )
  • Plos One : McPip1 Down-regulates Il-2 Ex... (by )
  • Plos One : Toll-like Receptor 2 and Toll... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.