World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Intragenomic Profiling Using Multicopy Genes ; the Rdna Internal Transcribed Spacer Sequences of the Freshwater Sponge Ephydatia Fluviatilis, Volume 8

By Bryk, Mary

Click here to view

Book Id: WPLBN0003950942
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Intragenomic Profiling Using Multicopy Genes ; the Rdna Internal Transcribed Spacer Sequences of the Freshwater Sponge Ephydatia Fluviatilis, Volume 8  
Author: Bryk, Mary
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Bryk, M. (n.d.). Plos One : Intragenomic Profiling Using Multicopy Genes ; the Rdna Internal Transcribed Spacer Sequences of the Freshwater Sponge Ephydatia Fluviatilis, Volume 8. Retrieved from http://worldebookfair.com/


Description
Description : Multicopy genes, like ribosomal RNA genes (rDNA), are widely used to describe and distinguish individuals. Despite concerted evolution that homogenizes a large number of rDNA gene copies, the presence of different gene variants within a genome has been reported. Characterization of an organism by defining every single variant of tens to thousands of rDNA repeat units present in a eukaryotic genome would be quite unreasonable. Here we provide an alternative approach for the characterization of a set of internal transcribed spacer sequences found within every rDNA repeat unit by implementing direct sequencing methodology. The prominent allelic variants and their relative amounts characterizing an individual can be described by a single sequencing electropherogram of the mixed amplicon containing the variants present within the genome. We propose a method for rational analysis of heterogeneity of multicopy genes by compiling a profile based on quantification of different sequence variants of the internal transcribed spacers of the freshwater sponge Ephydatia fluviatilis as an example. In addition to using conventional substitution analysis, we have developed a mathematical method, the proportion model method, to quantify the relative amounts of allelic variants of different length using data from direct sequencing of the heterogeneous amplicon. This method is based on determining the expected signal intensity values (corresponding to peak heights from the sequencing electropherogram) by sequencing clones from the same or highly similar amplicon and comparing hypothesized combinations against the values obtained by direct sequencing of the heterogeneous amplicon. This method allowed to differentiate between all specimens analysed.

 

Click To View

Additional Books


  • Plos One : a Dna ‘barcode Blitz’ ; Rapid... (by )
  • Plos One : Mir-219-5P Inhibits Receptor ... (by )
  • Plos One : Evaluation of Adam-12 as a Di... (by )
  • Plos One : Factors Influencing the Estim... (by )
  • Plos One : Sleep Extension Normalizes Er... (by )
  • Plos One : Two Variants of the C-reactiv... (by )
  • Plos One : Social Factors and Leukocyte ... (by )
  • Plos One : Global Microrna Profiling of ... (by )
  • Plos One : Cortical Thickness Abnormalit... (by )
  • Plos One : Mating Signals Indicating Sex... (by )
  • Plos One : the N-terminal Β-sheet of Per... (by )
  • Plos One : Antibodies Against Marinobact... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.