World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Post-embryonic Transcriptomes of the Prawn MacRobrachium Rosenbergii ; Multigenic Succession Through Metamorphosis, Volume 7

By Yu, Jr-kai, Sky

Click here to view

Book Id: WPLBN0003958467
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Post-embryonic Transcriptomes of the Prawn MacRobrachium Rosenbergii ; Multigenic Succession Through Metamorphosis, Volume 7  
Author: Yu, Jr-kai, Sky
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Yu, J. S. (n.d.). Plos One : Post-embryonic Transcriptomes of the Prawn MacRobrachium Rosenbergii ; Multigenic Succession Through Metamorphosis, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : Like many metazoans, the freshwater prawn Macrobrachium rosenbergii begins its post-embryonic life with a set of morphologically distinct planktonic larval stages, followed by a benthic post-larval stage during which the maturing organism differs from the larvae both ecologically and physiologically. Understanding of the molecular basis underlying morphogenesis in crustaceans is limited to the observation that methyl farnesoate, the non-epoxidated form of the insect juvenile hormone, acts as the active crustacean juvenoid. Molt steroids were also linked to morphogenesis and several other molecular pathways, such as Hedgehog and Wnt, are known to underlie morphogenesis in all metazoans examined and, as such, are thought to do the same in crustaceans. Using next generation sequencing, we deep-sequenced the transcriptomes of several larval and post-larval stages. De novo assembly, followed by bioinformatics analysis, revealed that many novel transcripts are over-expressed in either larvae- or post-larvae-stage prawn, shedding light on the molecular basis underlying M. rosenbergii metamorphosis. Fast larval molting rates and periodic morphological changes were reflected in over-expression of transcripts annotated to the cell cycle, DNA replication and morphogenic pathways (i.e., Hedgehog and Wnt). Further characterization of transcripts assigned to morphogenic pathways by real-time RT-PCR reconfirmed their over-expression in larvae, albeit with a more complex expression pattern when examined in the individual developmental stages. The expression level of an orthologue of cytochrome P450, 15A1, known to epoxidize methyl farnesoate in insects, was increased in the late larval and early post-larval stages, in accordance with the role of methyl farnesoate in crustacean metamorphosis. This study exemplifies the applicability of a high-throughput sequencing approach for studying complex traits, including metamorphosis, providing new insight into this unexplored area of crustacean research.

 

Click To View

Additional Books


  • Plos One : Preimplantation Genetic Diagn... (by )
  • Plos One : Clinical Signs of Radiologic ... (by )
  • Plos One : in Vivo Mr Imaging of Intraar... (by )
  • Plos One : a Longitudinal Study of Motor... (by )
  • Plos One : Regulation of Two Renal Chlor... (by )
  • Plos One : Antigen Experience Shapes Phe... (by )
  • Plos One : an in Vivo C. Elegans Model S... (by )
  • Plos One : Acute Hypersensitivity of Plu... (by )
  • Plos One : Parental Influences on Pathog... (by )
  • Plos One : Retinal Nerve Fibre Layer Thi... (by )
  • Plos One : Nociceptin Induces Hypophagia... (by )
  • Plos One : Expression Analysis of Taste ... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.