World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency Through Rational Design, Volume 7

By Silman, Israel

Click here to view

Book Id: WPLBN0003959145
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency Through Rational Design, Volume 7  
Author: Silman, Israel
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Silman, I. (n.d.). Plos One : Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency Through Rational Design, Volume 7. Retrieved from http://worldebookfair.com/


Description
Description : Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little or no green chromophore are most useful. In this study, we used rational design to convert a yellow fluorescent mPlum mutant to a red-emitting RFP without reverting any of the mutations causing the maturation deficiency and without altering the red chromophore’s covalent structure. We also created an optimized mPlum mutant (mPlum-E16P) that matures almost exclusively to the red chromophore. Analysis of the structure/function relationships in these proteins revealed two structural characteristics that are important for efficient red chromophore maturation in DsRed-derived RFPs. The first is the presence of a lysine residue at position 70 that is able to interact directly with the chromophore. The second is an absence of non-bonding interactions limiting the conformational flexibility at the peptide backbone that is oxidized during red chromophore formation. Satisfying or improving these structural features in other maturation-deficient RFPs may result in RFPs with faster and more complete maturation to the red chromophore.

 

Click To View

Additional Books


  • Plos One : Turning up the Heat ; Increas... (by )
  • Plos One : an Rna Interference Lethality... (by )
  • Plos One : Hiv-1 Envelope Glycoprotein V... (by )
  • Plos One : United States Acculturation a... (by )
  • Plos One : Oxygen Tension Modulates Diff... (by )
  • Plos One : Frequency Dependent Alteratio... (by )
  • Plos One : Quantitative Phenotyping of D... (by )
  • Plos One : Quality Control Methods in Ac... (by )
  • Plos One : Structural and Functional Stu... (by )
  • Plos One : Dysferlin-peptides Reallocate... (by )
  • Plos One : Women with Multiple Chemical ... (by )
  • Plos One : Doxycycline Alters Metabolism... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Fair are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.